Specimen SS-F-UMA-340 // UMAMI · IFI-U
Free Glutamate Accumulation III-BL-B
Solanum lycopersicum
Tomato
Target Trait
3.62 mg/L
mg/L
Free glutamate concentration
Technical Specifications
SKU
SS-F-UMA-340
Genomic Edits
8
GC Content
46.5-52.5%
Construct Length
4,313 bp
Promoter System
CaMV 35S / Ubiquitin
Expression System
Biolistic particle bombardment
Host Strain
Particle Gun
Receptor Target
mGluR1 / TAS1R1-TAS1R3
IFI Score
362
Pathway Edits
- SlGDH2 glutamate dehydrogenase isoform 2 overexpression under fruit-ripening RIN promoter for TCA-to-glutamate reductive amination burst at red stage
- SlGS1 cytosolic glutamine synthetase feedback-resistant mutant (G295A) for unregulated glutamine-to-glutamate cycling under high-nitrogen conditions
- SlGOGAT glutamine:2-oxoglutarate aminotransferase upregulation for expanded GS-GOGAT cycle flux amplifying net free glutamate output
- SlGLN1;2 pericarp-specific glutamine synthetase overexpression for localized free amino acid accumulation in fruit skin
- SlAAT aspartate aminotransferase isoform 1 redirected via active-site mutation (K256R) favoring glutamate over aspartate output
- SlVGT1 vacuolar glutamate transporter overexpression for apoplastic glutamate accumulation and controlled release upon maceration
- SlGABA-T GABA transaminase partial suppression (50% via miRNA) to reduce GABA shunt diversion from free glutamate pool
- SlNAD-ME NADP-dependent malic enzyme upregulation for enhanced malate-to-OAA conversion feeding alpha-ketoglutarate-to-glutamate pathway
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Fresh-market and processing tomatoes where elevated free glutamate drives MSG-free savory intensity. Applicable to tomato juice, concentrate, and passata for restaurant and food-service clients targeting natural umami labeling.
License
$1549 · Tier III License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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