Nucleotide Synergy IV-IV-B

Technical Specifications

Pathway Edits

• ▸GmADSS adenylosuccinate synthase overexpression for elevated IMP via de-novo purine pathway in Glycine max seed tissue
• ▸GmIMPDH2 inosine monophosphate dehydrogenase balanced overexpression for IMP/GMP co-accumulation without net IMP depletion
• ▸GmGMPS GMP synthase activity tuned to 60% of IMPDH output to maintain 1.7:1 IMP:GMP ratio for maximum synergy
• ▸GmADSL adenylosuccinate lyase upregulation ensuring AMP/IMP cycling prevents adenylate deficiency during IMP accumulation phase
• ▸GmNT5C 5′-nucleotidase expression calibrated via synthetic promoter strength to maintain free nucleotide fraction over phosphorylated forms
• ▸GmPNP purine nucleoside phosphorylase isoform 2 overexpression for nucleoside salvage amplification across IMP and GMP branches
• ▸GmGDH glutamate dehydrogenase upregulation for co-production of free glutamate to enhance nucleotide synergy via triple co-agonism
• ▸GmAMPD AMP deaminase partial knockdown (40%) to prevent IMP loss via adenylate catabolism during seed maturation
• ▸GmUMPS UMP synthase redirection variant for pyrimidine overflow suppression, maintaining carbon flux in purine pathway
• ▸GmHPRT hypoxanthine phosphoribosyltransferase overexpression for hypoxanthine salvage feeding IMP pool in protein storage vacuoles
• ▸GmGPRT guanine phosphoribosyltransferase upregulation for guanine-to-GMP salvage complementing de-novo GMP flux

Key Interventions