Nucleotide Synergy III-BL-A

Glycine max

Soybean

Tier IIINucleotide Synergy

Target Trait

3.75×

fold-change

IMP + GMP combined nucleotide synergy score

Technical Specifications

SKU

SS-F-UMA-299

Genomic Edits

GC Content

44.9-50.9%

Construct Length

4,416 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic particle bombardment

Host Strain

Particle Gun

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

375

Pathway Edits

GmADSS adenylosuccinate synthase overexpression for elevated IMP via de-novo purine pathway in Glycine max seed tissue
GmIMPDH2 inosine monophosphate dehydrogenase balanced overexpression for IMP/GMP co-accumulation without net IMP depletion
GmGMPS GMP synthase activity tuned to 60% of IMPDH output to maintain 1.7:1 IMP:GMP ratio for maximum synergy
GmADSL adenylosuccinate lyase upregulation ensuring AMP/IMP cycling prevents adenylate deficiency during IMP accumulation phase
GmNT5C 5′-nucleotidase expression calibrated via synthetic promoter strength to maintain free nucleotide fraction over phosphorylated forms
GmPNP purine nucleoside phosphorylase isoform 2 overexpression for nucleoside salvage amplification across IMP and GMP branches
GmGDH glutamate dehydrogenase upregulation for co-production of free glutamate to enhance nucleotide synergy via triple co-agonism
GmAMPD AMP deaminase partial knockdown (40%) to prevent IMP loss via adenylate catabolism during seed maturation

Key Interventions

Synergistic 5′-IMP + 5′-GMP co-accumulation at optimized 1.7:1 ratio for maximum mGluR1 and TAS1R1/TAS1R3 receptor co-activationTriple co-agonism via IMP + GMP + free glutamate co-engineering for IFI-U score multiplication beyond individual nucleotide effectsPurine pathway flux balancing preventing adenylate pool depletion while sustaining maximal IMP/GMP steady-state concentrations

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

375

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Soy-based umami concentrates, plant-based meat flavor systems, and fermented tofu products where dual-nucleotide synergy delivers maximum umami without exogenous additives. Ideal for functional food ingredient manufacturers.

License

$1499 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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