IFI-U · Nucleotide Synergy

Specimen SS-F-UMA-296 // UMAMI · IFI-U

Nucleotide Synergy II-IV-B

Glycine max

Soybean

Tier IINucleotide Synergy

Target Trait

3.31×

fold-change

IMP + GMP combined nucleotide synergy score

Technical Specifications

SKU

SS-F-UMA-296

Genomic Edits

8

GC Content

47.3-53.3%

Construct Length

4,334 bp

Promoter System

T7/SP6 cell-free

Expression System

Cell-free T7/SP6 in-vitro

Host Strain

Cell-free

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

331

Pathway Edits

  • GmADSS adenylosuccinate synthase overexpression for elevated IMP via de-novo purine pathway in Glycine max seed tissue
  • GmIMPDH2 inosine monophosphate dehydrogenase balanced overexpression for IMP/GMP co-accumulation without net IMP depletion
  • GmGMPS GMP synthase activity tuned to 60% of IMPDH output to maintain 1.7:1 IMP:GMP ratio for maximum synergy
  • GmADSL adenylosuccinate lyase upregulation ensuring AMP/IMP cycling prevents adenylate deficiency during IMP accumulation phase
  • GmNT5C 5′-nucleotidase expression calibrated via synthetic promoter strength to maintain free nucleotide fraction over phosphorylated forms
  • GmPNP purine nucleoside phosphorylase isoform 2 overexpression for nucleoside salvage amplification across IMP and GMP branches
  • GmGDH glutamate dehydrogenase upregulation for co-production of free glutamate to enhance nucleotide synergy via triple co-agonism
  • GmAMPD AMP deaminase partial knockdown (40%) to prevent IMP loss via adenylate catabolism during seed maturation

Key Interventions

Synergistic 5′-IMP + 5′-GMP co-accumulation at optimized 1.7:1 ratio for maximum mGluR1 and TAS1R1/TAS1R3 receptor co-activationTriple co-agonism via IMP + GMP + free glutamate co-engineering for IFI-U score multiplication beyond individual nucleotide effectsPurine pathway flux balancing preventing adenylate pool depletion while sustaining maximal IMP/GMP steady-state concentrations

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
331
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Soy-based umami concentrates, plant-based meat flavor systems, and fermented tofu products where dual-nucleotide synergy delivers maximum umami without exogenous additives. Ideal for functional food ingredient manufacturers.

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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