GMP Enhancement V-AG-A

Lentinus edodes

Shiitake

Tier VGMP Enhancement

Target Trait

3.8×

fold-change

Guanosine 5′-monophosphate (5′-GMP) accumulation

Technical Specifications

SKU

SS-F-UMA-273

Genomic Edits

GC Content

45.9-51.9%

Construct Length

4,436 bp

Promoter System

Tissue-specific 2A11 / CaMV35S

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

380

Pathway Edits

LeIMPDH2 inosine monophosphate dehydrogenase isoform 2 overexpression under gpd promoter for maximal IMP-to-XMP conversion feeding GMP branch
LeGMPS GMP synthase upregulation via synthetic promoter for high-flux XMP amination to GMP in Lentinus edodes fruiting bodies
LeGDH glutamate dehydrogenase overexpression for enhanced alpha-ketoglutarate reductive amination to glutamate, freeing GTP pool for GMP cycling
LeGTP-cyclohydrolase activity redirection from tetrahydrofolate toward GTP-surplus pathway to increase GTP availability for GMP re-synthesis
RNA-dependent GMP accumulation via endogenous RNA pool hydrolysis through LeRNase T2 upregulation in post-harvest drying phase
LePNP purine nucleoside phosphorylase engineered for preferential guanosine salvage to GMP over adenosine pathway
LeGMPK guanylate kinase feedback-resistant mutant (D77N) to uncouple GMP/GDP cycling and trap free GMP fraction
LeNT5′ 5′-nucleotidase overexpression to cycle GMP-to-guanosine and back via phosphorylation for elevated steady-state free GMP

Key Interventions

GMP-selective nucleotide engineering via IMPDH2/GMPS dual upregulation targeting TAS1R1/TAS1R3 secondary co-ligand slotPost-harvest GMP accumulation from RNA hydrolysis pathway activation for dried shiitake umami intensificationmGluR1 potentiation via GMP co-accumulation alongside free glutamate for synergistic IFI-U score elevation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

380

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Dried shiitake powder, mushroom extracts, and umami seasoning blends where high GMP content drives natural umami intensity equivalent to commercial yeast extracts. Suitable for clean-label soup bases and savory seasonings.

License

$9999 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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