IFI-U · GMP Enhancement

Specimen SS-F-UMA-261 // UMAMI · IFI-U

GMP Enhancement III-PR-A

Lentinus edodes

Shiitake

Tier IIIGMP Enhancement

Target Trait

2.55×

fold-change

Guanosine 5′-monophosphate (5′-GMP) accumulation

Technical Specifications

SKU

SS-F-UMA-261

Genomic Edits

6

GC Content

45.1-51.1%

Construct Length

3,610 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

255

Pathway Edits

  • LeIMPDH2 inosine monophosphate dehydrogenase isoform 2 overexpression under gpd promoter for maximal IMP-to-XMP conversion feeding GMP branch
  • LeGMPS GMP synthase upregulation via synthetic promoter for high-flux XMP amination to GMP in Lentinus edodes fruiting bodies
  • LeGDH glutamate dehydrogenase overexpression for enhanced alpha-ketoglutarate reductive amination to glutamate, freeing GTP pool for GMP cycling
  • LeGTP-cyclohydrolase activity redirection from tetrahydrofolate toward GTP-surplus pathway to increase GTP availability for GMP re-synthesis
  • RNA-dependent GMP accumulation via endogenous RNA pool hydrolysis through LeRNase T2 upregulation in post-harvest drying phase
  • LePNP purine nucleoside phosphorylase engineered for preferential guanosine salvage to GMP over adenosine pathway

Key Interventions

GMP-selective nucleotide engineering via IMPDH2/GMPS dual upregulation targeting TAS1R1/TAS1R3 secondary co-ligand slotPost-harvest GMP accumulation from RNA hydrolysis pathway activation for dried shiitake umami intensificationmGluR1 potentiation via GMP co-accumulation alongside free glutamate for synergistic IFI-U score elevation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
255
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Dried shiitake powder, mushroom extracts, and umami seasoning blends where high GMP content drives natural umami intensity equivalent to commercial yeast extracts. Suitable for clean-label soup bases and savory seasonings.

License

$1499 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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