IFI-U · GMP Enhancement

Specimen SS-F-UMA-254 // UMAMI · IFI-U

GMP Enhancement II-PR-B

Lentinus edodes

Shiitake

Tier IIGMP Enhancement

Target Trait

2.22×

fold-change

Guanosine 5′-monophosphate (5′-GMP) accumulation

Technical Specifications

SKU

SS-F-UMA-254

Genomic Edits

5

GC Content

46.8-52.8%

Construct Length

3,280 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

222

Pathway Edits

  • LeIMPDH2 inosine monophosphate dehydrogenase isoform 2 overexpression under gpd promoter for maximal IMP-to-XMP conversion feeding GMP branch
  • LeGMPS GMP synthase upregulation via synthetic promoter for high-flux XMP amination to GMP in Lentinus edodes fruiting bodies
  • LeGDH glutamate dehydrogenase overexpression for enhanced alpha-ketoglutarate reductive amination to glutamate, freeing GTP pool for GMP cycling
  • LeGTP-cyclohydrolase activity redirection from tetrahydrofolate toward GTP-surplus pathway to increase GTP availability for GMP re-synthesis
  • RNA-dependent GMP accumulation via endogenous RNA pool hydrolysis through LeRNase T2 upregulation in post-harvest drying phase

Key Interventions

GMP-selective nucleotide engineering via IMPDH2/GMPS dual upregulation targeting TAS1R1/TAS1R3 secondary co-ligand slotPost-harvest GMP accumulation from RNA hydrolysis pathway activation for dried shiitake umami intensificationmGluR1 potentiation via GMP co-accumulation alongside free glutamate for synergistic IFI-U score elevation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
222
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Dried shiitake powder, mushroom extracts, and umami seasoning blends where high GMP content drives natural umami intensity equivalent to commercial yeast extracts. Suitable for clean-label soup bases and savory seasonings.

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

Already purchased?

Back to Umami · IFI-UAll Modalities