Specimen SS-F-UMA-233 // UMAMI · IFI-U
IMP Biosynthesis V-AG-A
Solanum lycopersicum
Tomato
Target Trait
4×
fold-change
Inosine 5′-monophosphate (5′-IMP) accumulation
Technical Specifications
SKU
SS-F-UMA-233
Genomic Edits
9
GC Content
45.9-51.9%
Construct Length
4,736 bp
Promoter System
Tissue-specific 2A11 / CaMV35S
Expression System
Agrobacterium-mediated T-DNA
Host Strain
EHA105
Receptor Target
mGluR1 / TAS1R1-TAS1R3
IFI Score
400
Pathway Edits
- SlADSS adenylosuccinate synthase overexpression under CaMV35S promoter for de-novo IMP flux amplification via SAMP intermediate
- SlADSL adenylosuccinate lyase upregulation for SAMP-to-AMP conversion acceleration, expanding purine pool feeding IMP via AMPD
- SlHGPRT hypoxanthine-guanine phosphoribosyltransferase overexpression for salvage-pathway IMP recovery from hypoxanthine
- SlIMPDH inosine monophosphate dehydrogenase partial RNAi knockdown (60%) to prevent IMP oxidation to XMP, preserving free IMP pool
- SlPurA GMP reductase variant introduction to redirect GMP back to IMP under nucleotide surplus conditions
- SlXMP accumulation suppressed via SlGMPS guanosine monophosphate synthase down-regulation, reducing IMP diversion to GMP branch
- SlNT5C 5′-nucleotidase overexpression for enhanced IMP dephosphorylation and re-phosphorylation cycling to elevate free IMP steady state
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tomato products requiring clean-label umami enhancement via natural nucleotide co-agonists. Ideal for tomato paste, ketchup, and diced-tomato applications targeting MSG-free, IMP-driven savory intensity.
License
$9999 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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