IFI-U · IMP Biosynthesis

Specimen SS-F-UMA-230 // UMAMI · IFI-U

IMP Biosynthesis IV-PR-B

Solanum lycopersicum

Tomato

Tier IVIMP Biosynthesis

Target Trait

3.89×

fold-change

Inosine 5′-monophosphate (5′-IMP) accumulation

Technical Specifications

SKU

SS-F-UMA-230

Genomic Edits

9

GC Content

49.3-55.3%

Construct Length

4,654 bp

Promoter System

Ubiquitin / T7

Expression System

Protoplast PEG-fusion

Host Strain

PEG-mediated

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

389

Pathway Edits

  • SlADSS adenylosuccinate synthase overexpression under CaMV35S promoter for de-novo IMP flux amplification via SAMP intermediate
  • SlADSL adenylosuccinate lyase upregulation for SAMP-to-AMP conversion acceleration, expanding purine pool feeding IMP via AMPD
  • SlHGPRT hypoxanthine-guanine phosphoribosyltransferase overexpression for salvage-pathway IMP recovery from hypoxanthine
  • SlIMPDH inosine monophosphate dehydrogenase partial RNAi knockdown (60%) to prevent IMP oxidation to XMP, preserving free IMP pool
  • SlPurA GMP reductase variant introduction to redirect GMP back to IMP under nucleotide surplus conditions
  • SlXMP accumulation suppressed via SlGMPS guanosine monophosphate synthase down-regulation, reducing IMP diversion to GMP branch
  • SlNT5C 5′-nucleotidase overexpression for enhanced IMP dephosphorylation and re-phosphorylation cycling to elevate free IMP steady state

Key Interventions

mGluR1 co-agonist IMP pool maximization via de-novo purine biosynthesis plus salvage pathway dual amplificationTAS1R1/TAS1R3 synergist IMP accumulation targeting 5′-nucleotide receptor binding site for maximum umami potentiationIMPDH blockade preserving IMP from oxidative diversion toward XMP/GMP branch

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
389
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Tomato products requiring clean-label umami enhancement via natural nucleotide co-agonists. Ideal for tomato paste, ketchup, and diced-tomato applications targeting MSG-free, IMP-driven savory intensity.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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