IMP Biosynthesis I-BL-B

Solanum lycopersicum

Tomato

Tier IIMP Biosynthesis

Target Trait

1.72×

fold-change

Inosine 5′-monophosphate (5′-IMP) accumulation

Technical Specifications

SKU

SS-F-UMA-204

Genomic Edits

GC Content

47.1-53.1%

Construct Length

3,199 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic particle bombardment

Host Strain

Particle Gun

Receptor Target

mGluR1 / TAS1R1-TAS1R3

IFI Score

172

Pathway Edits

SlADSS adenylosuccinate synthase overexpression under CaMV35S promoter for de-novo IMP flux amplification via SAMP intermediate
SlADSL adenylosuccinate lyase upregulation for SAMP-to-AMP conversion acceleration, expanding purine pool feeding IMP via AMPD
SlHGPRT hypoxanthine-guanine phosphoribosyltransferase overexpression for salvage-pathway IMP recovery from hypoxanthine
SlIMPDH inosine monophosphate dehydrogenase partial RNAi knockdown (60%) to prevent IMP oxidation to XMP, preserving free IMP pool
SlPurA GMP reductase variant introduction to redirect GMP back to IMP under nucleotide surplus conditions

Key Interventions

mGluR1 co-agonist IMP pool maximization via de-novo purine biosynthesis plus salvage pathway dual amplificationTAS1R1/TAS1R3 synergist IMP accumulation targeting 5′-nucleotide receptor binding site for maximum umami potentiationIMPDH blockade preserving IMP from oxidative diversion toward XMP/GMP branch

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

172

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

—

IFI-A

—

IFI-F

—

Use Case

Tomato products requiring clean-label umami enhancement via natural nucleotide co-agonists. Ideal for tomato paste, ketchup, and diced-tomato applications targeting MSG-free, IMP-driven savory intensity.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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