IFI-P · Acrolein Mitigation Series

Specimen SS-F-TRP-976 // PUNGENT · IFI-P

Acrolein Mitigation Series II-IV-B

Nasturtium officinale

Watercress

Tier IIAcrolein Mitigation Series

Target Trait

1.49×

fold-change

Acrolein-analog volatile TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-976

Genomic Edits

4

GC Content

43.7%

Construct Length

2,774 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

149

Pathway Edits

  • LOX2 (lipoxygenase 2) upregulation for C18-fatty acid hydroperoxide formation in Nasturtium officinale leaf tissue
  • HYDROLYASE (fatty acid hydroperoxide hydrolyase) engineering for C3-volatile aldehyde cleavage product yield optimization
  • FAD (fatty acid desaturase) activity modulation for linolenic acid C18:3 substrate enrichment as LOX2 input
  • AOS (allene oxide synthase) partial knockdown to redirect FA hydroperoxide flux toward hydrolyase-mediated volatile pathway

Key Interventions

LOX2/HYDROLYASE cascade optimization for controlled C3-volatile aldehyde accumulation in Nasturtium officinale via linolenic acid oxidationAOS partial knockdown redirects fatty acid hydroperoxide flux from jasmonate pathway toward hydrolyase-mediated volatile pungent aldehyde formationAcrolein-analog TRPA1 volatile aldehyde agonism confirmed by IFI-P calcium-flux assay; mild irritating pungency characteristic of green-volatile leaf volatiles validated in panelCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
149
IFI-A
—
IFI-F
—

Use Case

Tier II acrolein-analog volatile platform for green-pungent flavor notes in Nasturtium-based condiments, watercress-flavor ingredient standardization, and low-intensity TRPA1 aldehyde activation in fresh salad dressings and cold-process sauces requiring the characteristic peppery-green sharpness of Nasturtium at calibrated sub-irritant concentrations verified by IFI-P panel scoring.

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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