Acrolein Mitigation Series I-AG-A

Nasturtium officinale

Watercress

Tier IAcrolein Mitigation Series

Target Trait

1×

fold-change

Acrolein-analog volatile TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-961

Genomic Edits

GC Content

45.8%

Construct Length

2,128 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

TRPA1

IFI Score

100

Pathway Edits

LOX2 (lipoxygenase 2) upregulation for C18-fatty acid hydroperoxide formation in Nasturtium officinale leaf tissue
HYDROLYASE (fatty acid hydroperoxide hydrolyase) engineering for C3-volatile aldehyde cleavage product yield optimization

Key Interventions

LOX2/HYDROLYASE cascade optimization for controlled C3-volatile aldehyde accumulation in Nasturtium officinale via linolenic acid oxidationAOS partial knockdown redirects fatty acid hydroperoxide flux from jasmonate pathway toward hydrolyase-mediated volatile pungent aldehyde formationAcrolein-analog TRPA1 volatile aldehyde agonism confirmed by IFI-P calcium-flux assay; mild irritating pungency characteristic of green-volatile leaf volatiles validated in panelT-DNA binary vector 2A11 system validated for tissue-specific stable genomic integration

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

100

IFI-A

—

IFI-F

—

Use Case

Tier I acrolein-analog volatile platform for green-pungent flavor notes in Nasturtium-based condiments, watercress-flavor ingredient standardization, and low-intensity TRPA1 aldehyde activation in fresh salad dressings and cold-process sauces requiring the characteristic peppery-green sharpness of Nasturtium at calibrated sub-irritant concentrations verified by IFI-P panel scoring.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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