IFI-P · Phenylacetaldehyde Series

Specimen SS-F-TRP-936 // PUNGENT · IFI-P

Phenylacetaldehyde Series II-IV-B

Brassica nigra

Black Mustard

Tier IIPhenylacetaldehyde Series

Target Trait

1.9×

fold-change

Phenylacetaldehyde TRPA1 aldehyde agonist fold-change

Technical Specifications

SKU

SS-F-TRP-936

Genomic Edits

5

GC Content

43.7%

Construct Length

3,274 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

190

Pathway Edits

  • PAR1 (phenylacetaldehyde reductase 1) downregulation to prevent phenylacetaldehyde reduction to 2-phenylethanol, maximizing aldehyde accumulation
  • PAAS (phenylacetaldehyde synthase / aromatic amino acid decarboxylase) overexpression for phenylalanine-to-phenylacetaldehyde one-step conversion in Brassica nigra
  • PAL downregulation in relevant tissue type to redirect phenylalanine from trans-cinnamic acid branch toward PAAS-mediated decarboxylative pathway
  • Phenylalanine aminomutase (PAM) modulation for phenylalanine pool management in PAAS-overexpression context
  • Vacuolar aldehyde sequestration transporter engineering for phenylacetaldehyde compartmentalization and controlled burst-release

Key Interventions

PAAS overexpression with PAR1 downregulation establishes high-yield phenylacetaldehyde accumulation in Brassica nigra through one-step phenylalanine decarboxylationPAL-branch partial suppression and phenylalanine transporter upregulation maximize PAAS substrate supply for phenylacetaldehyde productionPhenylacetaldehyde TRPA1 agonism via aldehyde electrophilic Schiff base formation at TRPA1 Lys708 confirmed by IFI-P calcium-flux assayCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
190
IFI-A
—
IFI-F
—

Use Case

Tier II phenylacetaldehyde-enriched Brassica platform for rose-honey flavor notes with TRPA1 mildly pungent character, floral-pungent flavor ingredient production in confectionery and baked goods, and functional flavor applications where the distinctive phenylacetaldehyde floral warmth complements mustard-sulfur or spice-heat profiles in complex savory-sweet product formulations.

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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