IFI-P · Dehydromatricarin Series

Specimen SS-F-TRP-920 // PUNGENT · IFI-P

Dehydromatricarin Series V-IV-B

Sinapis alba

White Mustard

Tier VDehydromatricarin Series

Target Trait

3.68×

fold-change

Dehydromatricarin sesquiterpene TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-920

Genomic Edits

8

GC Content

44.2%

Construct Length

4,435 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

368

Pathway Edits

  • Germacrene-D synthase (GDS) overexpression for C15-germacrene-D sesquiterpene scaffold biosynthesis in Sinapis alba
  • FPPS upregulation for FPP sesquiterpene precursor pool expansion in mustard metabolic background
  • CYP450 sesquiterpene lactone ring-forming cyclase for germacrene-D guaianolide lactone ring closure
  • Dehydrogenase enzyme for C2-C3 double bond introduction completing dehydromatricarin alpha-methylene-gamma-lactone pharmacophore
  • DXS/HMGR dual upregulation for MEP/MVA pathway isoprenoid precursor flux amplification
  • Sesquiterpene ABC transporter upregulation for cytoplasmic guaianolide lactone accumulation
  • Competing diterpene/triterpene pathway partial suppression for FPP flux concentration toward sesquiterpene lactone branch

Key Interventions

GDS/CYP450 sesquiterpene lactone cascade establishes dehydromatricarin guaianolide biosynthesis from FPP in Sinapis albaAlpha-methylene-gamma-lactone pharmacophore completion enables TRPA1 Michael addition at Cys621 for reactive sesquiterpene TRPA1 agonismDehydromatricarin TRPA1 agonism confirmed by IFI-P calcium-flux assay; bitter-pungent Asteraceae sesquiterpene sensory profile validated in panelCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
368
IFI-A
—
IFI-F
—

Use Case

Tier V dehydromatricarin-enriched platform for Asteraceae sesquiterpene TRPA1 agonist ingredient production, bitter-pungent flavor applications in herbal extracts, and research-grade guaianolide lactone supply for TRPA1 electrophile pharmacology. Suitable for flavor researchers exploring sesquiterpene lactone sensory axes and specialty bitter-pungent flavor ingredient developers for digestif, medicinal herb, and functional wellness product applications.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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