Specimen SS-F-TRP-880 // PUNGENT · IFI-P
4-HNE Series V-IV-B
Brassica nigra
Black Mustard
Target Trait
2.88×
fold-change
4-hydroxynonenal TRPA1 oxidative ligand fold-change
Technical Specifications
SKU
SS-F-TRP-880
Genomic Edits
7
GC Content
44.2%
Construct Length
3,975 bp
Promoter System
T7/SP6 cell-free
Expression System
In-vitro cell-free
Host Strain
Cell-free
Receptor Target
TRPA1
IFI Score
288
Pathway Edits
- LOX (lipoxygenase / 13-LOX) upregulation for linoleic acid oxidation to 13-hydroperoxy-octadecadienoic acid (13-HPODE) in Brassica nigra
- HPL (hydroperoxide lyase) overexpression for 13-HPODE cleavage to C9 aldehydes and 4-oxo-trans-2-nonenal intermediate
- ADH (alcohol dehydrogenase) modulation for C6/C9 volatile aldehyde chain-length selectivity in LOX-HPL pathway output
- Omega-3 fatty acid desaturase (FAD3) upregulation for linolenic acid pool expansion as LOX substrate for extended C9-aldehyde biosynthesis
- AOS (allene oxide synthase) competing pathway suppression to redirect 13-HPODE flux from jasmonate biosynthesis to HPL-mediated aldehyde formation
- Cytochrome P450-mediated 4-hydroxylation of nonenal precursor for 4-HNE analog biosynthesis
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Tier V 4-HNE-enriched platform for TRPA1 oxidative-sensing research applications, controlled oxidative note flavor development in cold-pressed oils and green vegetable extracts, and functional flavor ingredient production targeting the low-intensity green-fatty pungency associated with lipid-derived volatile aldehydes in cooked brassica and leafy vegetable products.
License
$10049 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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