IFI-P · 4-HNE Series

Specimen SS-F-TRP-875 // PUNGENT · IFI-P

4-HNE Series V-BL-A

Brassica nigra

Black Mustard

Tier V4-HNE Series

Target Trait

2.5×

fold-change

4-hydroxynonenal TRPA1 oxidative ligand fold-change

Technical Specifications

SKU

SS-F-TRP-875

Genomic Edits

6

GC Content

48.7%

Construct Length

3,510 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TRPA1

IFI Score

250

Pathway Edits

  • LOX (lipoxygenase / 13-LOX) upregulation for linoleic acid oxidation to 13-hydroperoxy-octadecadienoic acid (13-HPODE) in Brassica nigra
  • HPL (hydroperoxide lyase) overexpression for 13-HPODE cleavage to C9 aldehydes and 4-oxo-trans-2-nonenal intermediate
  • ADH (alcohol dehydrogenase) modulation for C6/C9 volatile aldehyde chain-length selectivity in LOX-HPL pathway output
  • Omega-3 fatty acid desaturase (FAD3) upregulation for linolenic acid pool expansion as LOX substrate for extended C9-aldehyde biosynthesis
  • AOS (allene oxide synthase) competing pathway suppression to redirect 13-HPODE flux from jasmonate biosynthesis to HPL-mediated aldehyde formation
  • Cytochrome P450-mediated 4-hydroxylation of nonenal precursor for 4-HNE analog biosynthesis

Key Interventions

LOX/HPL cascade amplification for C9-aldehyde 4-HNE-like TRPA1 oxidative ligand accumulation in Brassica nigraAOS competing pathway suppression redirects 13-HPODE hydroperoxide flux from jasmonate biosynthesis toward HPL-mediated 4-HNE-analog aldehyde formation4-HNE-class TRPA1 agonism via electrophilic Michael acceptor addition at Cys621/His719 confirmed by IFI-P calcium-flux assayBiolistic bombardment parameters optimized for high-copy tandem stable integration events

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
250
IFI-A
—
IFI-F
—

Use Case

Tier V 4-HNE-enriched platform for TRPA1 oxidative-sensing research applications, controlled oxidative note flavor development in cold-pressed oils and green vegetable extracts, and functional flavor ingredient production targeting the low-intensity green-fatty pungency associated with lipid-derived volatile aldehydes in cooked brassica and leafy vegetable products.

License

$9999 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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