4-HNE Series I-AG-A

Brassica nigra

Black Mustard

Tier I4-HNE Series

Target Trait

1.1×

fold-change

4-hydroxynonenal TRPA1 oxidative ligand fold-change

Technical Specifications

SKU

SS-F-TRP-841

Genomic Edits

GC Content

45.8%

Construct Length

2,468 bp

Promoter System

Tissue-specific 2A11

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

TRPA1

IFI Score

110

Pathway Edits

LOX (lipoxygenase / 13-LOX) upregulation for linoleic acid oxidation to 13-hydroperoxy-octadecadienoic acid (13-HPODE) in Brassica nigra
HPL (hydroperoxide lyase) overexpression for 13-HPODE cleavage to C9 aldehydes and 4-oxo-trans-2-nonenal intermediate
ADH (alcohol dehydrogenase) modulation for C6/C9 volatile aldehyde chain-length selectivity in LOX-HPL pathway output

Key Interventions

LOX/HPL cascade amplification for C9-aldehyde 4-HNE-like TRPA1 oxidative ligand accumulation in Brassica nigraAOS competing pathway suppression redirects 13-HPODE hydroperoxide flux from jasmonate biosynthesis toward HPL-mediated 4-HNE-analog aldehyde formation4-HNE-class TRPA1 agonism via electrophilic Michael acceptor addition at Cys621/His719 confirmed by IFI-P calcium-flux assayT-DNA binary vector 2A11 system validated for tissue-specific stable genomic integration

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

110

IFI-A

—

IFI-F

—

Use Case

Tier I 4-HNE-enriched platform for TRPA1 oxidative-sensing research applications, controlled oxidative note flavor development in cold-pressed oils and green vegetable extracts, and functional flavor ingredient production targeting the low-intensity green-fatty pungency associated with lipid-derived volatile aldehydes in cooked brassica and leafy vegetable products.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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