IFI-P · Isovelleral Series

Specimen SS-F-TRP-840 // PUNGENT · IFI-P

Isovelleral Series V-IV-B

Zingiber officinale

Ginger

Tier VIsovelleral Series

Target Trait

4.37×

fold-change

Isovelleral sesquiterpene TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-840

Genomic Edits

9

GC Content

44.2%

Construct Length

4,655 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

437

Pathway Edits

  • CYP88A6 (sesquiterpene hydroxylase) upregulation for germacrenol C-8 hydroxylation toward isovelleral skeleton in Zingiber officinale
  • Germacrenol synthase integration for germacrenol C15-skeleton biosynthesis as isovelleral precursor
  • FPPS upregulation for C15-FPP sesquiterpene precursor pool expansion in ginger metabolic background
  • CYP450 aldehyde-forming oxidation enzyme for isovelleral C-8 and C-9 dialdehyde completion
  • NAD(P)H oxidoreductase activity optimization for germacrenol to germacradienol oxidation intermediate
  • Sesquiterpene export transporter upregulation for cytoplasmic accumulation of isovelleral-precursor intermediates
  • Sterol biosynthesis competitor partial suppression for enhanced FPP flux toward sesquiterpene dialdehyde pathway
  • Transcription factor network modulation for sustained sesquiterpene pathway gene cluster expression

Key Interventions

CYP88A6/germacrenol synthase cascade engineering establishes isovelleral-class sesquiterpene dialdehyde biosynthesis in Zingiber officinale backgroundCYP450 aldehyde oxidation completes the reactive dialdehyde scaffold required for TRPA1 covalent Schiff base activation at Lys708/Lys969Isovelleral TRPA1 potency confirmed by IFI-P calcium-flux assay; pungent-irritating sesquiterpene activation profile validated in human sensory panelCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
437
IFI-A
—
IFI-F
—

Use Case

Tier V isovelleral-enriched platform for exotic TRPA1 agonist ingredient production, research-grade sesquiterpene dialdehyde supply for flavor receptor pharmacology, and specialty pungency applications in high-value culinary products requiring novel electrophilic warmth signatures distinct from conventional ITC or capsaicin pungency axes. Primarily suitable for flavor research institutions and specialty ingredient developers exploring sesquiterpene-class TRPA1 ligands.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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