IFI-P · Isovelleral Series

Specimen SS-F-TRP-811 // PUNGENT · IFI-P

Isovelleral Series II-BL-A

Zingiber officinale

Ginger

Tier IIIsovelleral Series

Target Trait

2.08×

fold-change

Isovelleral sesquiterpene TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-811

Genomic Edits

5

GC Content

49.1%

Construct Length

3,149 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TRPA1

IFI Score

208

Pathway Edits

  • CYP88A6 (sesquiterpene hydroxylase) upregulation for germacrenol C-8 hydroxylation toward isovelleral skeleton in Zingiber officinale
  • Germacrenol synthase integration for germacrenol C15-skeleton biosynthesis as isovelleral precursor
  • FPPS upregulation for C15-FPP sesquiterpene precursor pool expansion in ginger metabolic background
  • CYP450 aldehyde-forming oxidation enzyme for isovelleral C-8 and C-9 dialdehyde completion
  • NAD(P)H oxidoreductase activity optimization for germacrenol to germacradienol oxidation intermediate

Key Interventions

CYP88A6/germacrenol synthase cascade engineering establishes isovelleral-class sesquiterpene dialdehyde biosynthesis in Zingiber officinale backgroundCYP450 aldehyde oxidation completes the reactive dialdehyde scaffold required for TRPA1 covalent Schiff base activation at Lys708/Lys969Isovelleral TRPA1 potency confirmed by IFI-P calcium-flux assay; pungent-irritating sesquiterpene activation profile validated in human sensory panelBiolistic bombardment parameters optimized for high-copy tandem stable integration events

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
208
IFI-A
—
IFI-F
—

Use Case

Tier II isovelleral-enriched platform for exotic TRPA1 agonist ingredient production, research-grade sesquiterpene dialdehyde supply for flavor receptor pharmacology, and specialty pungency applications in high-value culinary products requiring novel electrophilic warmth signatures distinct from conventional ITC or capsaicin pungency axes. Primarily suitable for flavor research institutions and specialty ingredient developers exploring sesquiterpene-class TRPA1 ligands.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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