IFI-P · Polygodial Series

Specimen SS-F-TRP-800 // PUNGENT · IFI-P

Polygodial Series V-IV-B

Zingiber officinale

Ginger

Tier VPolygodial Series

Target Trait

4.6×

fold-change

Polygodial sesquiterpene TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-800

Genomic Edits

10

GC Content

44.2%

Construct Length

5,155 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

460

Pathway Edits

  • Germacrene synthase (GAS) overexpression for germacrene-D sesquiterpene scaffold biosynthesis as polygodial precursor in Zingiber officinale
  • ECS (epi-cedrol synthase-related sesquiterpene cyclase) modulation for polygodial-class drimane skeleton formation via drimanyl diphosphate cyclization
  • Farnesyl diphosphate synthase (FPPS) upregulation for C15-FPP sesquiterpene precursor pool expansion
  • DXS (1-deoxy-D-xylulose-5-phosphate synthase) upregulation for MEP-pathway isoprenoid precursor flux enhancement
  • HMGR (3-hydroxy-3-methylglutaryl-CoA reductase) upregulation for MVA-pathway FPP precursor supply in ginger background
  • CYP450 drimane oxidation enzyme for polygodial C-8 aldehyde group introduction from drimanyl scaffold
  • NAD(P)H-dependent oxidoreductase for C-7 aldehyde oxidation completing the dialdehyde functionality required for TRPA1 Schiff base formation
  • ABC transporter upregulation for sesquiterpene dialdehyde export from plastid to cytoplasm for tissue accumulation
  • Sterol pathway competitor suppression via squalene synthase partial knockdown for FPP flux redirection toward sesquiterpene biosynthesis

Key Interventions

GAS/ECS sesquiterpene cyclase cascade establishes polygodial drimane dialdehyde biosynthesis from farnesyl diphosphate in Zingiber officinaleCYP450 drimane oxidation and NAD(P)H-dependent aldehyde completion yield the reactive dialdehyde pharmacophore for TRPA1 Schiff base formation at Lys708 and adjacent electrophilic residuesPolygodial TRPA1 agonism confirmed by IFI-P calcium-flux assay; distinctive pungent-irritating sensory profile characteristic of Drimys winterii bark validated in human panelCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
460
IFI-A
—
IFI-F
—

Use Case

Tier V polygodial-enriched ingredient for exotic pungency applications in specialty condiments, South American flavor profiles inspired by Drimys winterii (winter's bark) traditional uses, and TRPA1 research-grade agonist production for electrophilic receptor pharmacology studies. Suitable for high-end culinary ingredient suppliers, botanical extract researchers, and flavor developers exploring sesquiterpene dialdehyde pungency modalities beyond the conventional isothiocyanate-capsaicin axis.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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