IFI-P · Piperine Analog Series

Specimen SS-F-TRP-727 // PUNGENT · IFI-P

Piperine Analog Series I-IV-A

Brassica nigra

Black Mustard

Tier IPiperine Analog Series

Target Trait

1.4×

fold-change

Piperine-like TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-727

Genomic Edits

4

GC Content

46%

Construct Length

2,910 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

140

Pathway Edits

  • PIAL (piperine-related alkaloid synthase) heterologous integration for piperine-analog amide bond formation from piperoyl-CoA in Brassica nigra
  • 4CL (4-coumarate:CoA ligase) upregulation for piperonyl/piperoyl-CoA thioester pool expansion
  • NOMT (norreticuline 7-O-methyltransferase-related) activity optimization for methylenedioxy bridge formation in piperonyl scaffold
  • PAL/C4H upregulation for hydroxycinnamic acid precursor supply feeding piperoyl-CoA pathway

Key Interventions

PIAL/4CL/NOMT heterologous pathway integration establishes piperine-like amide scaffold biosynthesis in Brassica nigra from phenylpropanoid precursorsBAHD acyltransferase piperidine-N acylation replicates the piperine TRPA1-active amide pharmacophore with characteristic pepper warmth and bioavailability-enhancing propertiesPiperine analog TRPA1 agonism confirmed by IFI-P calcium-flux assay; characteristic black pepper heat profile validated in sensory panel cohortCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
140
IFI-A
—
IFI-F
—

Use Case

Tier I piperine-analog ingredient for black pepper flavor application, TRPA1-selective sustained oral warmth in spice blends, piperine's known bioavailability-enhancement synergism in nutraceutical formulations, and standardized pepper-pungency platforms for manufacturers requiring consistent piperine-equivalent concentrations independent of Piper nigrum botanical supply variability and harvest-year quality fluctuations.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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