IFI-P · Methyl Salicylate Series

Specimen SS-F-TRP-720 // PUNGENT · IFI-P

Methyl Salicylate Series V-IV-B

Thymus vulgaris

Thyme

Tier VMethyl Salicylate Series

Target Trait

3.22×

fold-change

Methyl salicylate TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-720

Genomic Edits

8

GC Content

44.2%

Construct Length

4,435 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

322

Pathway Edits

  • BSMT1 (benzoic acid/salicylic acid carboxymethyltransferase) overexpression for SAM-dependent salicylate methylation to methyl salicylate in Thymus vulgaris
  • PBS3 (avrPphB susceptibility 3 / GH3.12 adenylase) upregulation for isochorismate-to-salicylate flux optimization
  • ICS1 (isochorismate synthase 1) upregulation for isochorismate precursor accumulation feeding PBS3-mediated salicylate biosynthesis
  • PAL-branch SA biosynthesis amplification as redundant flux source for salicylate production
  • SAM synthetase upregulation for S-adenosyl methionine methyl-donor cofactor supply to BSMT1
  • BA2H (benzoic acid 2-hydroxylase / CYP71A1) upregulation for benzoate-to-salicylate hydroxylation under PAL-branch pathway
  • UGT salicylate glucoside formation pathway suppression to maintain free salicylate pool as BSMT1 substrate

Key Interventions

BSMT1/PBS3/ICS1 cascade amplification for high-yield methyl salicylate production via dual salicylate pathway engineering in Thymus vulgarisSAM synthetase upregulation ensures adequate SAM cofactor supply for BSMT1-catalyzed methylation under production conditionsMethyl salicylate TRPA1 agonism confirmed by IFI-P calcium-flux assay; characteristic wintergreen-cooling pungency validated in sensory cohortCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
322
IFI-A
—
IFI-F
—

Use Case

Tier V methyl salicylate-enriched platform for wintergreen flavor ingredient production, TRPA1-selective cool-warm sensory contrast in confectionery, dental care product flavoring, and botanical extract standardization for beverages and functional food products requiring verifiable methyl salicylate concentrations independent of Betula or Gaultheria botanical supply variability.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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