IFI-P · Methyl Salicylate Series

Specimen SS-F-TRP-700 // PUNGENT · IFI-P

Methyl Salicylate Series III-BL-B

Thymus vulgaris

Thyme

Tier IIIMethyl Salicylate Series

Target Trait

2.18×

fold-change

Methyl salicylate TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-700

Genomic Edits

6

GC Content

45.1%

Construct Length

3,673 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TRPA1

IFI Score

218

Pathway Edits

  • BSMT1 (benzoic acid/salicylic acid carboxymethyltransferase) overexpression for SAM-dependent salicylate methylation to methyl salicylate in Thymus vulgaris
  • PBS3 (avrPphB susceptibility 3 / GH3.12 adenylase) upregulation for isochorismate-to-salicylate flux optimization
  • ICS1 (isochorismate synthase 1) upregulation for isochorismate precursor accumulation feeding PBS3-mediated salicylate biosynthesis
  • PAL-branch SA biosynthesis amplification as redundant flux source for salicylate production
  • SAM synthetase upregulation for S-adenosyl methionine methyl-donor cofactor supply to BSMT1
  • BA2H (benzoic acid 2-hydroxylase / CYP71A1) upregulation for benzoate-to-salicylate hydroxylation under PAL-branch pathway

Key Interventions

BSMT1/PBS3/ICS1 cascade amplification for high-yield methyl salicylate production via dual salicylate pathway engineering in Thymus vulgarisSAM synthetase upregulation ensures adequate SAM cofactor supply for BSMT1-catalyzed methylation under production conditionsMethyl salicylate TRPA1 agonism confirmed by IFI-P calcium-flux assay; characteristic wintergreen-cooling pungency validated in sensory cohortBiolistic bombardment parameters optimized for high-copy tandem stable integration events

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
218
IFI-A
—
IFI-F
—

Use Case

Tier III methyl salicylate-enriched platform for wintergreen flavor ingredient production, TRPA1-selective cool-warm sensory contrast in confectionery, dental care product flavoring, and botanical extract standardization for beverages and functional food products requiring verifiable methyl salicylate concentrations independent of Betula or Gaultheria botanical supply variability.

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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