IFI-P · Zingerone Series

Specimen SS-F-TRP-656 // PUNGENT · IFI-P

Zingerone Series II-IV-B

Zingiber officinale

Ginger

Tier IIZingerone Series

Target Trait

1.9×

fold-change

Zingerone TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-656

Genomic Edits

5

GC Content

43.7%

Construct Length

3,114 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

190

Pathway Edits

  • HCS (p-hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase) upregulation for vanillylacetone precursor flux in Zingiber officinale
  • pHBH (p-hydroxybenzaldehyde hydroxylase) upregulation for phenylbutanone intermediate transformation
  • CS (chalcone synthase-related) polyketide condensation optimization for vanillyl-acetone Claisen condensation product formation from feruloyl-CoA and malonyl-CoA
  • GAS (gingerdione synthase-related DKSI) modulation for gingerol dehydration to zingerone under heat treatment conditions
  • CCoAOMT optimization for feruloyl-CoA production from coumaroyl-CoA for vanillyl-side-chain assembly

Key Interventions

HCS/CS polyketide condensation pathway amplification for elevated gingerol precursor pool enabling efficient zingerone formation via thermal dehydration in Zingiber officinaleGAS/DKSI modulation optimizes zingerone yield from dehydration of [6]-gingerol substrate under processing-relevant temperaturesZingerone TRPA1 agonism confirmed by IFI-P calcium-flux assay; mild vanillyl-ketone warmth validated in sensory panel as characteristic cooked-ginger pungencyCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
190
IFI-A
—
IFI-F
—

Use Case

Tier II zingerone-enriched ginger ingredient for cooked-ginger flavor standardization, mild TRPA1 warming in baked goods and confectionery, and processed-ginger flavor applications in beverages, sauces, and ginger-forward condiments where the characteristic cooked-ginger pungency profile is preferred over the sharper raw gingerol or shogaol sensory attributes.

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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