Zingerone Series I-IV-A

Zingiber officinale

Ginger

Tier IZingerone Series

Target Trait

1.2×

fold-change

Zingerone TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-647

Genomic Edits

GC Content

46%

Construct Length

2,370 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

120

Pathway Edits

HCS (p-hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase) upregulation for vanillylacetone precursor flux in Zingiber officinale
pHBH (p-hydroxybenzaldehyde hydroxylase) upregulation for phenylbutanone intermediate transformation
CS (chalcone synthase-related) polyketide condensation optimization for vanillyl-acetone Claisen condensation product formation from feruloyl-CoA and malonyl-CoA

Key Interventions

HCS/CS polyketide condensation pathway amplification for elevated gingerol precursor pool enabling efficient zingerone formation via thermal dehydration in Zingiber officinaleGAS/DKSI modulation optimizes zingerone yield from dehydration of [6]-gingerol substrate under processing-relevant temperaturesZingerone TRPA1 agonism confirmed by IFI-P calcium-flux assay; mild vanillyl-ketone warmth validated in sensory panel as characteristic cooked-ginger pungencyCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

120

IFI-A

—

IFI-F

—

Use Case

Tier I zingerone-enriched ginger ingredient for cooked-ginger flavor standardization, mild TRPA1 warming in baked goods and confectionery, and processed-ginger flavor applications in beverages, sauces, and ginger-forward condiments where the characteristic cooked-ginger pungency profile is preferred over the sharper raw gingerol or shogaol sensory attributes.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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