IFI-P · Eugenol Series

Specimen SS-F-TRP-624 // PUNGENT · IFI-P

Eugenol Series III-IV-B

Thymus vulgaris

Thyme

Tier IIIEugenol Series

Target Trait

3.28×

fold-change

Eugenol TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-624

Genomic Edits

8

GC Content

44.3%

Construct Length

4,341 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

328

Pathway Edits

  • EGS1 (eugenol synthase 1) overexpression for coniferyl acetate reduction to eugenol in Thymus vulgaris
  • CCOMT (caffeoyl-CoA O-methyltransferase) upregulation for feruloyl-CoA production feeding coniferyl alcohol and acetate pathway
  • CCoAOMT (caffeoyl-CoA O-methyltransferase) optimization for 3-O-methylation of caffeoyl intermediates for eugenol scaffold
  • CAD (cinnamyl alcohol dehydrogenase) modulation for coniferyl alcohol accumulation as EGS1 substrate
  • C3H (coumarate 3-hydroxylase / CYP98A3) upregulation for p-coumaroyl to caffeoyl ester conversion in eugenol pathway
  • ATC (alcohol acyltransferase) optimization for coniferyl acetate assembly from coniferyl alcohol for EGS1 input
  • PAL upregulation for phenylalanine-to-trans-cinnamic acid flux amplification feeding eugenol phenylpropanoid backbone
  • Lignin branch CYP84A1-null allele introduction to redirect 5-hydroxyconiferyl flux toward eugenol biosynthesis

Key Interventions

EGS1/CCOMT/CCoAOMT cascade amplification for high-yield free eugenol accumulation via coniferyl acetate reductase pathway in Thymus vulgarisCAD/ATC coordination provides optimal coniferyl acetate substrate supply to EGS1 for maximum eugenol yield from phenylpropanoid precursor fluxEugenol TRPA1 agonism confirmed by IFI-P calcium-flux assay; characteristic warm, clove-spice pungency activating TRPA1 non-covalently validated in human sensory panelCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
328
IFI-A
—
IFI-F
—

Use Case

Tier III eugenol-enriched platform for clove-flavor ingredient production, TRPA1-selective spice warmth in baked goods, dental anesthetic flavor masking applications, and standardized eugenol supply for flavor houses requiring consistent clove-phenol concentrations in spice blends, mulled beverage preparations, and tropical condiment formulations.

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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