IFI-P · Carvacrol Series

Specimen SS-F-TRP-592 // PUNGENT · IFI-P

Carvacrol Series IV-IV-B

Origanum vulgare

Oregano

Tier IVCarvacrol Series

Target Trait

3.65×

fold-change

Carvacrol TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-592

Genomic Edits

8

GC Content

45.9%

Construct Length

4,428 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

365

Pathway Edits

  • CYP71D (carvacrol synthase P450) upregulation for para-cymene hydroxylation to carvacrol in Origanum vulgare
  • SABATH methyltransferase suppression to prevent carvacrol O-methylation to thymyl methyl ether byproduct
  • GPP synthase overexpression for expanded geranyl diphosphate pool feeding oregano monoterpene biosynthesis
  • Para-cymene synthase (CymS) upregulation for dedicated para-cymene substrate supply to CYP71D carvacrol hydroxylase
  • TPS (terpene synthase) regulatory network rebalancing for carvacrol over thymol pathway selectivity
  • CYP71A1 competing pathway suppression for monoterpene flux concentration in carvacrol-selective branch
  • Trichome-specific promoter engineering for glandular trichome-targeted carvacrol accumulation in leaf tissue
  • Vacuolar sequestration transporter upregulation for carvacrol storage and controlled sensory release

Key Interventions

CYP71D/para-cymene synthase cascade amplification for high-yield carvacrol biosynthesis in Origanum vulgare glandular trichomesSABATH methyltransferase suppression maintains free carvacrol in the phenol form required for TRPA1 non-covalent electrophilic activationCarvacrol TRPA1 agonism confirmed by IFI-P calcium-flux assay; characteristic warm, spicy-herbal oregano pungency profile validated in sensory panel cohortCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
365
IFI-A
—
IFI-F
—

Use Case

Tier IV carvacrol-enriched oregano ingredient platform for Mediterranean flavor formulation, pizza and tomato sauce flavor standardization, TRPA1-targeted herbal pungency in dressings and marinades, and functional food applications leveraging carvacrol's well-characterized antimicrobial and flavor properties. Suitable for manufacturers requiring consistent essential oil phenol content independent of Origanum vulgare geographic or seasonal chemotype variability.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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