IFI-P · Thymol Series

Specimen SS-F-TRP-552 // PUNGENT · IFI-P

Thymol Series IV-IV-B

Thymus vulgaris

Thyme

Tier IVThymol Series

Target Trait

3.43×

fold-change

Thymol TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-552

Genomic Edits

7

GC Content

45.9%

Construct Length

3,968 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

343

Pathway Edits

  • CYP71D180 (thymol synthase cytochrome P450) overexpression for gamma-terpinene hydroxylation to thymol in Thymus vulgaris
  • Thymol synthase regulatory element engineering for constitutive CYP71D180 expression independent of developmental stage
  • G10H (geraniol 10-hydroxylase) upregulation for monoterpene precursor geranyl diphosphate pool expansion
  • GPP synthase (geranyl pyrophosphate synthase) overexpression for thymol C10-skeleton precursor biosynthesis
  • Gamma-terpinene synthase (TvTPS1) upregulation for dedicated gamma-terpinene substrate supply to CYP71D180
  • CYP71A1 downregulation to suppress competing linalool hydroxylation pathway diverting monoterpene flux
  • OMT (O-methyltransferase) thymol-analog branch suppression to favor unsubstituted thymol over carvacrol or methyl-thymol products

Key Interventions

CYP71D180/G10H cascade amplification for high-yield thymol accumulation in Thymus vulgaris via gamma-terpinene substrate channelingGPP synthase overexpression expands the C10-monoterpene precursor pool feeding the dedicated thymol biosynthesis branch with minimal carvacrol co-formationThymol TRPA1 agonism at the electrophilic binding site confirmed by IFI-P calcium-flux assay; characteristic herbal-pungent medicinal peppery sensation validated in panel cohortCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
343
IFI-A
—
IFI-F
—

Use Case

Tier IV thymol-enriched Thymus platform for precision herbal flavor ingredient production, TRPA1-selective warming pungency in culinary herbs, medicinal-flavor functional food applications, and standardized thymol supply for antimicrobial flavor systems where natural source variability introduces inconsistent sensory profiles in oregano-thyme spice blends and Mediterranean flavor applications.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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