IFI-P · Thymol Series

Specimen SS-F-TRP-523 // PUNGENT · IFI-P

Thymol Series I-BL-A

Thymus vulgaris

Thyme

Tier IThymol Series

Target Trait

1.4×

fold-change

Thymol TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-523

Genomic Edits

3

GC Content

47.1%

Construct Length

2,462 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TRPA1

IFI Score

140

Pathway Edits

  • CYP71D180 (thymol synthase cytochrome P450) overexpression for gamma-terpinene hydroxylation to thymol in Thymus vulgaris
  • Thymol synthase regulatory element engineering for constitutive CYP71D180 expression independent of developmental stage
  • G10H (geraniol 10-hydroxylase) upregulation for monoterpene precursor geranyl diphosphate pool expansion

Key Interventions

CYP71D180/G10H cascade amplification for high-yield thymol accumulation in Thymus vulgaris via gamma-terpinene substrate channelingGPP synthase overexpression expands the C10-monoterpene precursor pool feeding the dedicated thymol biosynthesis branch with minimal carvacrol co-formationThymol TRPA1 agonism at the electrophilic binding site confirmed by IFI-P calcium-flux assay; characteristic herbal-pungent medicinal peppery sensation validated in panel cohortBiolistic bombardment parameters optimized for high-copy tandem stable integration events

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
140
IFI-A
—
IFI-F
—

Use Case

Tier I thymol-enriched Thymus platform for precision herbal flavor ingredient production, TRPA1-selective warming pungency in culinary herbs, medicinal-flavor functional food applications, and standardized thymol supply for antimicrobial flavor systems where natural source variability introduces inconsistent sensory profiles in oregano-thyme spice blends and Mediterranean flavor applications.

License

$299 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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