IFI-P · Alpha-Sanshool Series

Specimen SS-F-TRP-519 // PUNGENT · IFI-P

Alpha-Sanshool Series V-IV-A

Sinapis alba

White Mustard

Tier VAlpha-Sanshool Series

Target Trait

4×

fold-change

Alpha-sanshool alkylamide TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-519

Genomic Edits

8

GC Content

47.4%

Construct Length

4,418 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

400

Pathway Edits

  • FADS2 (fatty acid desaturase 2) upregulation for C16/C18 polyunsaturated fatty acid substrate production feeding alkylamide biosynthesis
  • Acyl-CoA synthetase (LACS) isoform overexpression for activation of sanshool-chain fatty acid intermediates
  • PKS-like polyketide synthase integration for alkenyl-chain assembly from C2-extension condensation cycles
  • Amide bond-forming enzyme (BAHD acyltransferase) introduction for N-isobutylamide conjugation to hydroxylated alkenyl chain
  • CYP450 chain-hydroxylation enzyme for sanshool-analog C-2 hydroxy group introduction
  • Fatty acid elongase (FAE1) modulation for extended alkylamide carbon-chain length optimization
  • Lipid transfer protein suppression to prevent sequestration of sanshool-analog intermediates in subcellular lipid bodies
  • Transcription factor network modulation for sustained alkylamide pathway gene expression under production conditions

Key Interventions

FADS2/acyl-CoA synthetase cascade amplification for alkylamide carbon-chain substrate production in Sinapis alba heterologous backgroundBAHD acyltransferase introduction enables N-isobutylamide conjugation replicating the sanshool tingling-numbing TRPA1/KCNK3 dual activation pharmacophoreAlpha-sanshool analog TRPA1 agonism confirmed by IFI-P calcium-flux assay; distinctive tingling-prickling oral sensation validated in sensory panel cohortCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
400
IFI-A
—
IFI-F
—

Use Case

Tier V alpha-sanshool-enriched ingredient platform for Sichuan pepper flavor applications, tingling-numbing mouthfeel development in culinary products, and TRPA1/KCNK3 dual-target sensory ingredient production for novel flavor texture experiences in sauces, noodles, and specialty condiments. Designed for flavor innovators seeking the authentic ma (numbing) sensation of Zanthoxylum without botanical supply constraints or regulatory complexity of whole spice imports.

License

$9999 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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