IFI-P · Propyl Disulfide Series

Specimen SS-F-TRP-472 // PUNGENT · IFI-P

Propyl Disulfide Series IV-IV-B

Allium sativum

Garlic

Tier IVPropyl Disulfide Series

Target Trait

3.14×

fold-change

Dipropyl disulfide TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-472

Genomic Edits

7

GC Content

45.9%

Construct Length

4,008 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

314

Pathway Edits

  • C-S lyase (cysteine-sulfoxide lyase) upregulation for propyl-cysteine sulfoxide cleavage and DPS precursor liberation
  • Propylcysteine sulfoxide (PCSO) biosynthesis pathway amplification via propyl-substituted cysteine S-oxidase
  • FMO3 (flavin-dependent monooxygenase 3) upregulation for S-oxidation of S-propyl-L-cysteine to propyl sulfoxide form
  • GGT-mediated gamma-glutamyl-S-propylcysteine processing pathway enhancement
  • Alliinase-family enzyme isoform with propyl-substrate specificity introduction for PCSO hydrolysis
  • Sulfur metabolite flux optimization via SAT/OAS-TL cysteine synthase complex upregulation
  • Thiosulfinate oxidation pathway suppression to stabilize dipropyl disulfide rather than disulfoxide products

Key Interventions

C-S lyase/PCSO pathway amplification for dipropyl disulfide (DPS) TRPA1 agonist accumulation in Allium sativum platformFMO3-driven propyl-cysteine S-oxidation maximizes PCSO precursor pool for C-S lyase substrate availabilityDPS TRPA1 activation confirmed by calcium-flux assay; dipropyl disulfide engages TRPA1 electrophilic binding site with characteristic onion-sulfur pungency profileCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
314
IFI-A
—
IFI-F
—

Use Case

Tier IV DPS-enriched Allium ingredient for cooked-onion and mild garlic flavor profiles in processed foods, TRPA1-selective sulfurous pungency in soups, stews, and savory condiments requiring a less pungent but warm, penetrating sulfur character. Suitable for applications where allicin's sharp raw-garlic volatility is undesirable but TRPA1-mediated warmth and sulfurous depth are targeted flavor attributes.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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