IFI-P · Allicin Engineering Series

Specimen SS-F-TRP-439 // PUNGENT · IFI-P

Allicin Engineering Series V-IV-A

Allium sativum

Garlic

Tier VAllicin Engineering Series

Target Trait

4.5×

fold-change

Allicin TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-439

Genomic Edits

9

GC Content

47.4%

Construct Length

4,638 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

450

Pathway Edits

  • Alliinase (alliin lyase) overexpression for enhanced S-allyl-L-cysteine sulfoxide (alliin) hydrolysis and allicin generation
  • ACSO lyase isoform pathway amplification for allyl cysteine sulfoxide biosynthesis from gamma-glutamyl precursors
  • CS pathway (cysteine synthase) flux optimization for allyl-cysteine sulfoxide precursor supply
  • GGT (gamma-glutamyl transpeptidase) enhancement for gamma-glutamyl-S-allylcysteine processing to alliin
  • FMO (flavin-dependent monooxygenase) upregulation for S-oxidation of allyl cysteine to alliin sulfoxide form
  • Vacuolar alliin storage compartment engineering for substrate-enzyme separation and burst-release kinetics
  • CSE (cystathionine gamma-lyase) modulation for sulfur flux optimization in allicin biosynthetic pathway
  • AlliA promoter engineering for constitutive alliinase expression in transformed Allium sativum lines
  • SAT (serine acetyltransferase) upregulation for cysteine pool expansion feeding CS-pathway allyl-cysteine biosynthesis

Key Interventions

Alliinase/ACSO lyase cascade amplification for high-yield allicin (diallyl thiosulfinate) generation upon tissue disruption in Allium sativumFMO-driven S-oxidation enhancement maximizes alliin precursor accumulation in vacuolar compartments for controlled allicin burst-releaseAllicin TRPA1 electrophilic agonism via thiosulfinate-mediated cysteine modification at TRPA1 Cys621/Cys641 confirmed by IFI-P calcium-flux assayCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
450
IFI-A
—
IFI-F
—

Use Case

Tier V allicin-enriched Allium platform for precision garlic flavor ingredient production, TRPA1-targeted pungency in savory condiments, and standardized allicin supply for functional food and nutraceutical applications where conventional garlic allicin variability introduces formulation inconsistency. Suitable for sauce manufacturers, processed food developers, and culinary ingredient suppliers requiring verifiable allicin concentrations with defined sensory impact.

License

$9999 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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