IFI-P · Allicin Engineering Series

Specimen SS-F-TRP-415 // PUNGENT · IFI-P

Allicin Engineering Series II-IV-A

Allium sativum

Garlic

Tier IIAllicin Engineering Series

Target Trait

2.33×

fold-change

Allicin TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-415

Genomic Edits

5

GC Content

47.3%

Construct Length

3,217 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

233

Pathway Edits

  • Alliinase (alliin lyase) overexpression for enhanced S-allyl-L-cysteine sulfoxide (alliin) hydrolysis and allicin generation
  • ACSO lyase isoform pathway amplification for allyl cysteine sulfoxide biosynthesis from gamma-glutamyl precursors
  • CS pathway (cysteine synthase) flux optimization for allyl-cysteine sulfoxide precursor supply
  • GGT (gamma-glutamyl transpeptidase) enhancement for gamma-glutamyl-S-allylcysteine processing to alliin
  • FMO (flavin-dependent monooxygenase) upregulation for S-oxidation of allyl cysteine to alliin sulfoxide form

Key Interventions

Alliinase/ACSO lyase cascade amplification for high-yield allicin (diallyl thiosulfinate) generation upon tissue disruption in Allium sativumFMO-driven S-oxidation enhancement maximizes alliin precursor accumulation in vacuolar compartments for controlled allicin burst-releaseAllicin TRPA1 electrophilic agonism via thiosulfinate-mediated cysteine modification at TRPA1 Cys621/Cys641 confirmed by IFI-P calcium-flux assayCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
233
IFI-A
—
IFI-F
—

Use Case

Tier II allicin-enriched Allium platform for precision garlic flavor ingredient production, TRPA1-targeted pungency in savory condiments, and standardized allicin supply for functional food and nutraceutical applications where conventional garlic allicin variability introduces formulation inconsistency. Suitable for sauce manufacturers, processed food developers, and culinary ingredient suppliers requiring verifiable allicin concentrations with defined sensory impact.

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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