IFI-P · Erucin Series

Specimen SS-F-TRP-392 // PUNGENT · IFI-P

Erucin Series IV-IV-B

Brassica nigra

Black Mustard

Tier IVErucin Series

Target Trait

3.68×

fold-change

Erucin TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-392

Genomic Edits

7

GC Content

45.9%

Construct Length

4,048 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

368

Pathway Edits

  • GSTU20 (glutathione S-transferase) upregulation for glucoerucin biosynthesis and TRPA1-active erucin formation
  • MAM3 (methylthioalkylmalate synthase 3) overexpression for 4-methylthiobutyl glucosinolate chain elongation
  • CYP79F1 upregulation for long-chain aliphatic glucosinolate oxime synthesis from homomethionine
  • FMOGS-OX1-null allele introduction to prevent glucoerucin oxidation to sulforaphane-analog branch
  • TGG1 myrosinase optimization for glucoerucin hydrolysis and free erucin release kinetics
  • AOP2 suppression for erucin ITC pathway selectivity over gluconapin-forming branch
  • AtST5a sulfotransferase upregulation for high-flux desulfo-glucoerucin processing

Key Interventions

MAM3/CYP79F1 chain-elongation pathway amplification for glucoerucin pool expansion in Brassica nigraFMOGS-OX1-null allele preserves glucoerucin in the reduced thioether form for selective erucin ITC formation via TGG1 myrosinaseErucin TRPA1 electrophilic agonism confirmed by IFI-P calcium-flux assay; erucin isothiocyanate targets TRPA1 Cys621 for covalent channel activationCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
368
IFI-A
—
IFI-F
—

Use Case

Tier IV erucin-enriched Brassica platform for sulfurous-ITC flavor applications in mustard condiments, cooked brassica flavor development, and TRPA1-selective functional ingredient production where erucin's milder, less volatile profile relative to AITC is preferred for thermal stability in hot-processed food applications.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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