IFI-P · PEITC Series

Specimen SS-F-TRP-336 // PUNGENT · IFI-P

PEITC Series II-IV-B

Nasturtium officinale

Watercress

Tier IIPEITC Series

Target Trait

3.16×

fold-change

Phenethyl isothiocyanate TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-336

Genomic Edits

6

GC Content

43.7%

Construct Length

3,654 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

316

Pathway Edits

  • CYP79A1 (phenylalanine N-hydroxylase) upregulation for phenethyl glucosinolate oxime biosynthesis from L-phenylalanine
  • BGLU23 (beta-glucosidase myrosinase) enhancement for gluconasturtiin hydrolysis and PEITC liberation
  • CYP83B1 N-hydroxy-phenylacetaldoxime dehydratase upregulation for aromatic thiohydroximate intermediate flux
  • UGT74B1 glucosyltransferase efficiency optimization for gluconasturtiin assembly rate
  • AOP3-null allele introduction to prevent gluconasturtiin oxidative sidechain conversion
  • ESP-like protein knockdown for selective PEITC formation over nitrile analog

Key Interventions

CYP79A1/BGLU23 cascade amplification for high-yield PEITC liberation from gluconasturtiin in Nasturtium officinaleAOP3-null allele combined with ESP knockdown ensures near-exclusive PEITC formation with minimal non-pungent sidechain product formationPAL-driven phenylalanine precursor expansion feeds CYP79A1 for sustained PEITC production under variable growth conditionsCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
316
IFI-A
—
IFI-F
—

Use Case

Tier II PEITC-enriched Nasturtium platform for high-potency peppery-mustard flavor formulation, TRPA1-targeted functional ingredient development, and standardized PEITC supply for nutraceutical and chemopreventive food product lines. Designed for manufacturers seeking consistent phenethyl ITC concentrations independent of environmental variability in glucosinolate-producing crop systems.

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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