IFI-P · Benzyl ITC Series

Specimen SS-F-TRP-320 // PUNGENT · IFI-P

Benzyl ITC Series V-IV-B

Nasturtium officinale

Watercress

Tier VBenzyl ITC Series

Target Trait

4.83×

fold-change

Benzyl isothiocyanate TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-320

Genomic Edits

9

GC Content

44.2%

Construct Length

4,815 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

483

Pathway Edits

  • CYP79A2 (phenylalanine N-monooxygenase) overexpression for benzyl glucosinolate oxime biosynthesis from phenylalanine
  • UGT74C1 (glucosyltransferase) upregulation for glucobenzyl glucosinolate assembly and storage efficiency
  • TGG1 myrosinase integration for glucobenzyl hydrolysis and BITC release upon tissue disruption
  • ESP knockdown to minimize nitrile and episulfide byproduct formation from benzyl glucosinolate
  • CYP83B1 (N-hydroxy-benzylaldoxime synthase) upregulation for aromatic glucosinolate intermediate flux
  • SUR1 C-S lyase integration for thiohydroximate-to-glucosinolate conversion efficiency in Nasturtium
  • MYB34 transcription factor upregulation for aromatic glucosinolate pathway transcriptional reinforcement
  • Vacuolar GLT1-like transporter enhancement for glucobenzyl glucosinolate storage and controlled release

Key Interventions

CYP79A2-driven benzyl glucosinolate pathway amplification for BITC-selective TRPA1 electrophilic activation in Nasturtium officinaleESP knockdown combined with TGG1 myrosinase integration maximizes BITC yield and minimizes non-pungent nitrile formationAromatic glucosinolate-to-BITC cascade validated by TRPA1 calcium-flux assay with IFI-P subscale scoringCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
483
IFI-A
—
IFI-F
—

Use Case

Tier V BITC-enriched Nasturtium ingredient for peppery-mustard flavor applications, watercress-flavor concentrate production, and TRPA1-selective electrophilic pungency in dressings, fresh salad ingredients, and specialty condiments. Suitable for culinary ingredient manufacturers and functional food developers targeting the sharp, nasal-trigeminal activation signature of authentic fresh Nasturtium without supply-chain agronomic constraints.

License

$10049 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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