IFI-P · Benzyl ITC Series

Specimen SS-F-TRP-308 // PUNGENT · IFI-P

Benzyl ITC Series IV-BL-B

Nasturtium officinale

Watercress

Tier IVBenzyl ITC Series

Target Trait

4.14×

fold-change

Benzyl isothiocyanate TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-308

Genomic Edits

8

GC Content

43.4%

Construct Length

4,400 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TRPA1

IFI Score

414

Pathway Edits

  • CYP79A2 (phenylalanine N-monooxygenase) overexpression for benzyl glucosinolate oxime biosynthesis from phenylalanine
  • UGT74C1 (glucosyltransferase) upregulation for glucobenzyl glucosinolate assembly and storage efficiency
  • TGG1 myrosinase integration for glucobenzyl hydrolysis and BITC release upon tissue disruption
  • ESP knockdown to minimize nitrile and episulfide byproduct formation from benzyl glucosinolate
  • CYP83B1 (N-hydroxy-benzylaldoxime synthase) upregulation for aromatic glucosinolate intermediate flux
  • SUR1 C-S lyase integration for thiohydroximate-to-glucosinolate conversion efficiency in Nasturtium
  • MYB34 transcription factor upregulation for aromatic glucosinolate pathway transcriptional reinforcement
  • Vacuolar GLT1-like transporter enhancement for glucobenzyl glucosinolate storage and controlled release

Key Interventions

CYP79A2-driven benzyl glucosinolate pathway amplification for BITC-selective TRPA1 electrophilic activation in Nasturtium officinaleESP knockdown combined with TGG1 myrosinase integration maximizes BITC yield and minimizes non-pungent nitrile formationAromatic glucosinolate-to-BITC cascade validated by TRPA1 calcium-flux assay with IFI-P subscale scoringBiolistic bombardment parameters optimized for high-copy tandem stable integration events

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
414
IFI-A
—
IFI-F
—

Use Case

Tier IV BITC-enriched Nasturtium ingredient for peppery-mustard flavor applications, watercress-flavor concentrate production, and TRPA1-selective electrophilic pungency in dressings, fresh salad ingredients, and specialty condiments. Suitable for culinary ingredient manufacturers and functional food developers targeting the sharp, nasal-trigeminal activation signature of authentic fresh Nasturtium without supply-chain agronomic constraints.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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