IFI-P · Benzyl ITC Series

Specimen SS-F-TRP-292 // PUNGENT · IFI-P

Benzyl ITC Series II-BL-B

Nasturtium officinale

Watercress

Tier IIBenzyl ITC Series

Target Trait

2.76×

fold-change

Benzyl isothiocyanate TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-292

Genomic Edits

6

GC Content

44.6%

Construct Length

3,506 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TRPA1

IFI Score

276

Pathway Edits

  • CYP79A2 (phenylalanine N-monooxygenase) overexpression for benzyl glucosinolate oxime biosynthesis from phenylalanine
  • UGT74C1 (glucosyltransferase) upregulation for glucobenzyl glucosinolate assembly and storage efficiency
  • TGG1 myrosinase integration for glucobenzyl hydrolysis and BITC release upon tissue disruption
  • ESP knockdown to minimize nitrile and episulfide byproduct formation from benzyl glucosinolate
  • CYP83B1 (N-hydroxy-benzylaldoxime synthase) upregulation for aromatic glucosinolate intermediate flux
  • SUR1 C-S lyase integration for thiohydroximate-to-glucosinolate conversion efficiency in Nasturtium

Key Interventions

CYP79A2-driven benzyl glucosinolate pathway amplification for BITC-selective TRPA1 electrophilic activation in Nasturtium officinaleESP knockdown combined with TGG1 myrosinase integration maximizes BITC yield and minimizes non-pungent nitrile formationAromatic glucosinolate-to-BITC cascade validated by TRPA1 calcium-flux assay with IFI-P subscale scoringBiolistic bombardment parameters optimized for high-copy tandem stable integration events

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
276
IFI-A
—
IFI-F
—

Use Case

Tier II BITC-enriched Nasturtium ingredient for peppery-mustard flavor applications, watercress-flavor concentrate production, and TRPA1-selective electrophilic pungency in dressings, fresh salad ingredients, and specialty condiments. Suitable for culinary ingredient manufacturers and functional food developers targeting the sharp, nasal-trigeminal activation signature of authentic fresh Nasturtium without supply-chain agronomic constraints.

License

$749 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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