Methyl ITC Series I-IV-B

Sinapis alba

White Mustard

Tier IMethyl ITC Series

Target Trait

1.38×

fold-change

Methyl isothiocyanate TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-248

Genomic Edits

GC Content

45.2%

Construct Length

2,767 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

138

Pathway Edits

BCAT (branched-chain amino acid aminotransferase) upregulation for methionine chain-elongation pathway priming
CYP79B2 (methylthioalkyl glucosinolate oxime synthase) overexpression for methylated glucosinolate oxime biosynthesis
MYR2 myrosinase isoform integration for methyl glucosinolate-specific hydrolysis kinetics
AOP1 (2-oxoglutarate-dependent dioxygenase) suppression to prevent MITC precursor sidechain oxidation

Key Interventions

CYP79B2-driven methylated glucosinolate oxime amplification for MITC-selective TRPA1 activation in Sinapis albaMYR2 myrosinase specificity engineering for near-complete methyl glucosinolate hydrolysis with minimal side-product formationAOP1 suppression preserves MITC precursor integrity throughout post-harvest processing conditionsCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S

—

IFI-U

—

IFI-B

—

IFI-K

—

IFI-C

—

IFI-P

138

IFI-A

—

IFI-F

—

Use Case

Tier I MITC-enriched ingredient for mustard-family pungency applications, soil fumigation-inspired flavor analogues in culinary contexts, and TRPA1-targeted sensory activation in savory and fermented food formats. Designed for flavor manufacturers requiring consistent MITC yield from a stabilized White Mustard platform independent of seasonal precursor variability.

License

$349 · Tier I License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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