IFI-P · Cinnamaldehyde Series

Specimen SS-F-TRP-232 // PUNGENT · IFI-P

Cinnamaldehyde Series IV-IV-B

Cinnamomum verum

Cinnamon

Tier IVCinnamaldehyde Series

Target Trait

3.89×

fold-change

trans-cinnamaldehyde TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-232

Genomic Edits

8

GC Content

45.9%

Construct Length

4,308 bp

Promoter System

T7/SP6 cell-free

Expression System

In-vitro cell-free

Host Strain

Cell-free

Receptor Target

TRPA1

IFI Score

389

Pathway Edits

  • PAL (phenylalanine ammonia-lyase) overexpression for phenylalanine-to-trans-cinnamic acid flux amplification
  • C4H (cinnamate 4-hydroxylase / CYP73A) upregulation for hydroxycinnamic acid branch optimization
  • Cinnamoyl-CoA reductase (CCR1) activity enhancement for cinnamoyl substrate pool expansion
  • CAD (cinnamyl alcohol dehydrogenase) downregulation to redirect flux away from lignin toward aldehyde accumulation
  • CCoAOMT (caffeoyl-CoA O-methyltransferase) suppression for unsubstituted cinnamaldehyde selectivity
  • COMT (caffeic acid O-methyltransferase) knockdown to prevent methylated analog accumulation
  • 4CL (4-coumarate:CoA ligase) isoform selection for trans-cinnamoyl-CoA thioester preferential loading
  • Vacuolar export transporter upregulation for volatile cinnamaldehyde compartmentalization and burst-release

Key Interventions

PAL/C4H/CCR cascade amplification for high-flux trans-cinnamaldehyde accumulation in Cinnamomum verumCAD downregulation redirects phenylpropanoid flux from lignin to free aldehyde pool targeting TRPA1 electrophilic cysteine residuesVolatile cinnamaldehyde accumulation confirmed by TRPA1 calcium-flux assay with IFI-P panel scoringCell-free T7/SP6 translation system for in-vitro pungency pathway enzyme validation

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
389
IFI-A
—
IFI-F
—

Use Case

Tier IV cinnamaldehyde-enriched cinnamon ingredient platform for precision spice flavor formulation, TRPA1-targeted warming sensations in confectionery and bakery applications, and botanical extract standardization for nutraceutical and functional food products. Suitable for manufacturers requiring verifiable trans-cinnamaldehyde specifications independent of geographic source variability in Cinnamomum bark supply chains.

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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