IFI-P · Cinnamaldehyde Series

Specimen SS-F-TRP-220 // PUNGENT · IFI-P

Cinnamaldehyde Series III-BL-B

Cinnamomum verum

Cinnamon

Tier IIICinnamaldehyde Series

Target Trait

3.16×

fold-change

trans-cinnamaldehyde TRPA1 agonist fold-change

Technical Specifications

SKU

SS-F-TRP-220

Genomic Edits

7

GC Content

45.1%

Construct Length

3,893 bp

Promoter System

CaMV 35S / Ubiquitin

Expression System

Biolistic transformation

Host Strain

Particle Gun

Receptor Target

TRPA1

IFI Score

316

Pathway Edits

  • PAL (phenylalanine ammonia-lyase) overexpression for phenylalanine-to-trans-cinnamic acid flux amplification
  • C4H (cinnamate 4-hydroxylase / CYP73A) upregulation for hydroxycinnamic acid branch optimization
  • Cinnamoyl-CoA reductase (CCR1) activity enhancement for cinnamoyl substrate pool expansion
  • CAD (cinnamyl alcohol dehydrogenase) downregulation to redirect flux away from lignin toward aldehyde accumulation
  • CCoAOMT (caffeoyl-CoA O-methyltransferase) suppression for unsubstituted cinnamaldehyde selectivity
  • COMT (caffeic acid O-methyltransferase) knockdown to prevent methylated analog accumulation
  • 4CL (4-coumarate:CoA ligase) isoform selection for trans-cinnamoyl-CoA thioester preferential loading

Key Interventions

PAL/C4H/CCR cascade amplification for high-flux trans-cinnamaldehyde accumulation in Cinnamomum verumCAD downregulation redirects phenylpropanoid flux from lignin to free aldehyde pool targeting TRPA1 electrophilic cysteine residuesVolatile cinnamaldehyde accumulation confirmed by TRPA1 calcium-flux assay with IFI-P panel scoringBiolistic bombardment parameters optimized for high-copy tandem stable integration events

IFI Subscale Profile

IFI Profile

IFI-S
—
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
316
IFI-A
—
IFI-F
—

Use Case

Tier III cinnamaldehyde-enriched cinnamon ingredient platform for precision spice flavor formulation, TRPA1-targeted warming sensations in confectionery and bakery applications, and botanical extract standardization for nutraceutical and functional food products. Suitable for manufacturers requiring verifiable trans-cinnamaldehyde specifications independent of geographic source variability in Cinnamomum bark supply chains.

License

$1549 · Tier III License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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