Specimen SS-F-SWT-992 // SWEET · IFI-S
TAS1R2/R3 Allosteric Enhancement Series IV-IV-B
Solanum lycopersicum
Tomato
Target Trait
3.38×
fold-change
TAS1R2/R3 response fold-change
Technical Specifications
SKU
SS-F-SWT-992
Genomic Edits
7
GC Content
45.9%
Construct Length
4,048 bp
Promoter System
T7/SP6 promoter
Expression System
Cell-free transcription/translation
Host Strain
T7 RNAP + wheat germ extract
Receptor Target
TAS1R2/TAS1R3
IFI Score
338
Pathway Edits
- OBP1 odorant binding protein-like overexpression for endogenous TAS1R allosteric modulator production
- SW-TF sweetness-associated transcription factor overexpression for receptor density amplification
- SEP1 signal enhancement protein 1 integration for downstream TAS1R2/TAS1R3 signaling amplification
- TAS1R-modulator small molecule accumulation cassette for endogenous receptor potentiator synthesis
- Gustducin alpha subunit co-expression for enhanced G-protein coupling efficiency in tomato
- PLCbeta2 phospholipase C beta-2 upregulation for amplified IP3-mediated calcium release cascade
- TRPM5 channel expression element for enhanced transient receptor potential melastatin 5 activity
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Enhanced performance variant: regulatory metabolite documentation for allosteric enhancer-enriched tomato sweetness engineering with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications
License
$4049 · Tier IV License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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