IFI-S · TAS1R2/R3 Allosteric Enhancement Series

Specimen SS-F-SWT-991 // SWEET · IFI-S

TAS1R2/R3 Allosteric Enhancement Series IV-IV-A

Solanum lycopersicum

Tomato

Tier IVTAS1R2/R3 Allosteric Enhancement Series

Target Trait

3.38×

fold-change

TAS1R2/R3 response fold-change

Technical Specifications

SKU

SS-F-SWT-991

Genomic Edits

7

GC Content

46.2%

Construct Length

4,031 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

338

Pathway Edits

  • OBP1 odorant binding protein-like overexpression for endogenous TAS1R allosteric modulator production
  • SW-TF sweetness-associated transcription factor overexpression for receptor density amplification
  • SEP1 signal enhancement protein 1 integration for downstream TAS1R2/TAS1R3 signaling amplification
  • TAS1R-modulator small molecule accumulation cassette for endogenous receptor potentiator synthesis
  • Gustducin alpha subunit co-expression for enhanced G-protein coupling efficiency in tomato
  • PLCbeta2 phospholipase C beta-2 upregulation for amplified IP3-mediated calcium release cascade
  • TRPM5 channel expression element for enhanced transient receptor potential melastatin 5 activity

Key Interventions

OBP1 and TAS1R-modulator co-expression for endogenous allosteric receptor potentiator accumulationSW-TF sweetness transcription factor overexpression for TAS1R2/TAS1R3 receptor density enhancementSEP1 integration for amplified downstream TAS1R2/TAS1R3 signaling cascade response in tomato

IFI Subscale Profile

IFI Profile

IFI-S
338
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

regulatory metabolite documentation for allosteric enhancer-enriched tomato sweetness engineering

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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