Specimen SS-F-SWT-960 // SWEET · IFI-S
Erythritol Biosynthesis Series V-IV-B
Saccharum officinarum
Sugarcane
Target Trait
2.5×
fold-change
Erythritol fold-change
Technical Specifications
SKU
SS-F-SWT-960
Genomic Edits
6
GC Content
44.2%
Construct Length
3,555 bp
Promoter System
T7/SP6 promoter
Expression System
Cell-free transcription/translation
Host Strain
T7 RNAP + wheat germ extract
Receptor Target
TAS1R2/TAS1R3
IFI Score
250
Pathway Edits
- ERY1 erythrose reductase overexpression for direct erythrose-to-erythritol conversion in sugarcane
- TKL1 transketolase upregulation for increased erythrose-4-phosphate intermediate availability
- XKS xylulokinase co-expression for expanded pentose phosphate pathway flux toward erythrose
- GCY1 glycerol dehydrogenase expression for NADPH-dependent erythritol pathway completion
- Erythritol phosphate phosphatase integration for free erythritol release from phosphorylated intermediate
- Competing mannitol pathway suppression to redirect polyol biosynthesis toward erythritol production
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Enhanced performance variant: industrial erythritol engineering in sugarcane for scalable natural zero-calorie polyol manufacturing with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications
License
$10049 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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