IFI-S · Erythritol Biosynthesis Series

Specimen SS-F-SWT-952 // SWEET · IFI-S

Erythritol Biosynthesis Series IV-IV-B

Saccharum officinarum

Sugarcane

Tier IVErythritol Biosynthesis Series

Target Trait

2.13×

fold-change

Erythritol fold-change

Technical Specifications

SKU

SS-F-SWT-952

Genomic Edits

5

GC Content

45.9%

Construct Length

3,208 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

213

Pathway Edits

  • ERY1 erythrose reductase overexpression for direct erythrose-to-erythritol conversion in sugarcane
  • TKL1 transketolase upregulation for increased erythrose-4-phosphate intermediate availability
  • XKS xylulokinase co-expression for expanded pentose phosphate pathway flux toward erythrose
  • GCY1 glycerol dehydrogenase expression for NADPH-dependent erythritol pathway completion
  • Erythritol phosphate phosphatase integration for free erythritol release from phosphorylated intermediate
  • Competing mannitol pathway suppression to redirect polyol biosynthesis toward erythritol production

Key Interventions

ERY1 erythrose reductase overexpression for direct conversion of erythrose to erythritolTKL1 transketolase upregulation expanding erythrose-4-phosphate pentose phosphate pathway fluxXKS co-expression enhancing pentose phosphate intermediate supply for erythritol precursor availability

IFI Subscale Profile

IFI Profile

IFI-S
213
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

Enhanced performance variant: regulatory metabolite documentation for erythritol-enriched sugarcane as a natural low-calorie sweetener with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications

License

$4049 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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