Specimen SS-F-SWT-920 // SWEET · IFI-S
Curculin Taste-Modifying Series V-IV-B
Capsicum annuum
Pepper
Target Trait
5.5
IFI score
Curculin taste-modifying protein IFI accumulation
Technical Specifications
SKU
SS-F-SWT-920
Genomic Edits
7
GC Content
44.2%
Construct Length
4,055 bp
Promoter System
T7/SP6 promoter
Expression System
Cell-free transcription/translation
Host Strain
T7 RNAP + wheat germ extract
Receptor Target
TAS1R2/TAS1R3
IFI Score
550
Pathway Edits
- CURC-I curculin-I codon-optimized expression cassette for heterologous production in Capsicum
- CURC-II curculin-II isoform co-expression for optimal curculin homodimer or heterodimer assembly
- 35S-promoter element for constitutive high-level curculin expression in Capsicum
- Signal peptide optimization for curculin apoplastic targeting and enhanced stability in planta
- BiP and PDI chaperone co-expression for correct curculin lectin-domain disulfide formation
- Vacuolar targeting signal for alternative subcellular compartmentalization of curculin homodimer
- mRNA 3-UTR stability element for extended curculin transcript longevity in Capsicum tissue
Key Interventions
IFI Subscale Profile
IFI Profile
Use Case
Enhanced performance variant: industrial curculin production in pepper for scalable taste-conversion functional food manufacturing with amplified TAS1R2/TAS1R3 receptor activation for premium-tier applications
License
$10049 · Tier V License
Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.
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