IFI-S · Curculin Taste-Modifying Series

Specimen SS-F-SWT-919 // SWEET · IFI-S

Curculin Taste-Modifying Series V-IV-A

Capsicum annuum

Pepper

Tier VCurculin Taste-Modifying Series

Target Trait

5.5

IFI score

Curculin taste-modifying protein IFI accumulation

Technical Specifications

SKU

SS-F-SWT-919

Genomic Edits

7

GC Content

47.4%

Construct Length

4,038 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

550

Pathway Edits

  • CURC-I curculin-I codon-optimized expression cassette for heterologous production in Capsicum
  • CURC-II curculin-II isoform co-expression for optimal curculin homodimer or heterodimer assembly
  • 35S-promoter element for constitutive high-level curculin expression in Capsicum
  • Signal peptide optimization for curculin apoplastic targeting and enhanced stability in planta
  • BiP and PDI chaperone co-expression for correct curculin lectin-domain disulfide formation
  • Vacuolar targeting signal for alternative subcellular compartmentalization of curculin homodimer
  • mRNA 3-UTR stability element for extended curculin transcript longevity in Capsicum tissue

Key Interventions

CURC-I and CURC-II co-expression for optimal curculin homodimer assembly in Capsicum annuum35S-driven constitutive expression for high-level curculin taste-modifying protein accumulationSignal peptide optimization for apoplastic targeting and enhanced curculin protein stability

IFI Subscale Profile

IFI Profile

IFI-S
550
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

industrial curculin production in pepper for scalable taste-conversion functional food manufacturing

License

$9999 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

Already purchased?

Back to Sweet · IFI-SAll Modalities