IFI-S · Curculin Taste-Modifying Series

Specimen SS-F-SWT-911 // SWEET · IFI-S

Curculin Taste-Modifying Series IV-IV-A

Capsicum annuum

Pepper

Tier IVCurculin Taste-Modifying Series

Target Trait

4.63

IFI score

Curculin taste-modifying protein IFI accumulation

Technical Specifications

SKU

SS-F-SWT-911

Genomic Edits

6

GC Content

46.2%

Construct Length

3,491 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

463

Pathway Edits

  • CURC-I curculin-I codon-optimized expression cassette for heterologous production in Capsicum
  • CURC-II curculin-II isoform co-expression for optimal curculin homodimer or heterodimer assembly
  • 35S-promoter element for constitutive high-level curculin expression in Capsicum
  • Signal peptide optimization for curculin apoplastic targeting and enhanced stability in planta
  • BiP and PDI chaperone co-expression for correct curculin lectin-domain disulfide formation
  • Vacuolar targeting signal for alternative subcellular compartmentalization of curculin homodimer
  • mRNA 3-UTR stability element for extended curculin transcript longevity in Capsicum tissue

Key Interventions

CURC-I and CURC-II co-expression for optimal curculin homodimer assembly in Capsicum annuum35S-driven constitutive expression for high-level curculin taste-modifying protein accumulationSignal peptide optimization for apoplastic targeting and enhanced curculin protein stability

IFI Subscale Profile

IFI Profile

IFI-S
463
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

regulatory documentation of curculin as a natural taste-modifying protein for functional food filings

License

$3999 · Tier IV License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

Already purchased?

Back to Sweet · IFI-SAll Modalities