IFI-S · Curculin Taste-Modifying Series

Specimen SS-F-SWT-889 // SWEET · IFI-S

Curculin Taste-Modifying Series II-AG-A

Capsicum annuum

Pepper

Tier IICurculin Taste-Modifying Series

Target Trait

2.88

IFI score

Curculin taste-modifying protein IFI accumulation

Technical Specifications

SKU

SS-F-SWT-889

Genomic Edits

4

GC Content

47.8%

Construct Length

2,895 bp

Promoter System

35S CaMV promoter

Expression System

Agrobacterium-mediated T-DNA

Host Strain

EHA105

Receptor Target

TAS1R2/TAS1R3

IFI Score

288

Pathway Edits

  • CURC-I curculin-I codon-optimized expression cassette for heterologous production in Capsicum
  • CURC-II curculin-II isoform co-expression for optimal curculin homodimer or heterodimer assembly
  • 35S-promoter element for constitutive high-level curculin expression in Capsicum
  • Signal peptide optimization for curculin apoplastic targeting and enhanced stability in planta
  • BiP and PDI chaperone co-expression for correct curculin lectin-domain disulfide formation

Key Interventions

CURC-I and CURC-II co-expression for optimal curculin homodimer assembly in Capsicum annuum35S-driven constitutive expression for high-level curculin taste-modifying protein accumulationSignal peptide optimization for apoplastic targeting and enhanced curculin protein stability

IFI Subscale Profile

IFI Profile

IFI-S
288
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

pilot-scale curculin production for sour-sweet taste conversion functional ingredient formulation

License

$699 · Tier II License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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