IFI-S · Mabinlin Expression Series

Specimen SS-F-SWT-879 // SWEET · IFI-S

Mabinlin Expression Series V-IV-A

Saccharum officinarum

Sugarcane

Tier VMabinlin Expression Series

Target Trait

7.5

IFI score

Mabinlin sweet protein IFI accumulation

Technical Specifications

SKU

SS-F-SWT-879

Genomic Edits

7

GC Content

47.4%

Construct Length

3,958 bp

Promoter System

T7/SP6 promoter

Expression System

Cell-free transcription/translation

Host Strain

T7 RNAP + wheat germ extract

Receptor Target

TAS1R2/TAS1R3

IFI Score

750

Pathway Edits

  • MAB-II-codon codon-optimized mabinlin II expression cassette for heterologous production in sugarcane
  • 35S enhancer-driven promoter element for constitutive high-level mabinlin II expression
  • Signal peptide fusion for apoplastic targeting and enhanced mabinlin II protein stability
  • BiP chaperone co-expression for correct mabinlin II disulfide bridge formation and folding
  • Protease inhibitor PR-1 co-expression to protect accumulated mabinlin II from proteolysis
  • mRNA stability element insertion for extended mabinlin II transcript half-life in sugarcane
  • Protein stabilizing osmolyte accumulation element for enhanced mabinlin thermostability

Key Interventions

MAB-II codon-optimized transgene integration for high-yield mabinlin II sweet protein in sugarcaneSignal peptide engineering for apoplastic targeting and enhanced mabinlin II protein stability35S enhancer-driven transcriptional amplification for maximum mabinlin II expression output

IFI Subscale Profile

IFI Profile

IFI-S
750
IFI-U
—
IFI-B
—
IFI-K
—
IFI-C
—
IFI-P
—
IFI-A
—
IFI-F
—

Use Case

industrial mabinlin production for scalable heat-stable sweet protein food ingredient manufacturing

License

$9999 · Tier V License

Includes full construct sequence, CRISPR guide designs, pathway edit specifications, flux balance analysis, and IFI validation data.

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